Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR

Due to the genetic mutation (fa) in the gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase...

Full description

Bibliographic Details
Published in:	Heliyon
Main Authors:	Xinyun Xu, Xinge Hu, Guodong Ma, Tiannan Wang, Jayne Wu, Xiaojuan Zhu, Guoxun Chen, Ling Zhao, Jiangang Chen
Format:	Article
Language:	English
Published:	Elsevier 2023-09-01
Subjects:	fa mutation Leptin receptor Tetra-primer amplification-refractory mutation system (ARMS) Zucker rats Obesity
Online Access:	http://www.sciencedirect.com/science/article/pii/S240584402307367X

Similar Items

Tetra-Primer Amplification-Refractory Mutation System (ARMS)—PCR for Genotyping Mouse Leptin Gene Mutation
by: Jiangang Chen, et al.
Published: (2022-10-01)

Pioglitazone is effective for multiple phenotyepes of the Zucker fa/fa rat with polycystc ovary morphology and insulin resistance
by: Miyuki Morishita, et al.
Published: (2018-03-01)

Processing Alaska Pollock Protein (<i>Theragra chalcogramma</i>) into Kamaboko Protein Mitigates Elevated Serum Cholesterol and Postprandial Glucose Levels in Obese Zucker <i>fa/fa</i> Rats
by: Natsuka Takada, et al.
Published: (2022-10-01)

Polymorphisms within the <i>Boule</i> Gene Detected by Tetra-Primer Amplification Refractory Mutation System PCR (T-ARMS-PCR) are Significantly Associated with Goat Litter Size
by: Xiaoyue Song, et al.
Published: (2019-11-01)

Adiponectin negatively correlated with carotid arterial structure in the leptin-resistant Zucker diabetic fatty rat
by: Emmanuel Cosson, et al.
Published: (2011-08-01)

Reduction in the Dietary VA Status Prevents Type 2 Diabetes and Obesity in Zucker Diabetic Fatty Rats
by: Tiannan Wang, et al.
Published: (2022-03-01)

Development of tetra-primer allele specific PCR (tetra-primer AS-PCR) for the detection of L1014F mutation in sodium channel gene associated with pyrethroid resistance in mosquito Culex quinquefasciatus
by: Saowanee Chamnanya, et al.
Published: (2016-09-01)

Is energy expenditure reduced in obese mice with mutations in the leptin/leptin receptor genes?
by: Paul Trayhurn, et al.
Published: (2020-01-01)

Na,K-ATPase Kinetics and Oxidative Stress in Kidneys of Zucker Diabetic Fatty (fa/fa) Rats Depending on the Diabetes Severity—Comparison with Lean (fa/+) and Wistar Rats
by: Norbert Vrbjar, et al.
Published: (2022-10-01)

Development of SNP markers using RNA-seq technology and tetra-primer ARMS-PCR in sweetpotato
by: Meng KOU, et al.
Published: (2017-02-01)

Oxidative Stress Markers and Na,K-ATPase Enzyme Kinetics Are Altered in the Cerebellum of Zucker Diabetic Fatty fa/fa Rats: A Comparison with Lean fa/+ and Wistar Rats
by: Dominika Radosinska, et al.
Published: (2024-09-01)

Clinical Utility of Amplification Refractory Mutation System-Based PCR and Mutation-Specific PCR for Precise and Rapid Genotyping of Angiotensin-Converting Enzyme 1 (ACE1-rs4646996 D>I) and Angiotensin-Converting Enzyme 2 (ACE2-rs4240157T>C) Gene Variations in Coronary Artery Disease and Their Strong Association with Its Disease Susceptibility and Progression
by: Aadil Yousif, et al.
Published: (2022-05-01)

Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia
by: Silvio Pires Gomes, et al.
Published: (2025-03-01)

Tetra-primer amplification refractory mutation system-polymerase chain reaction (TARMS-PCR) assay in genotyping of single nucleotide polymorphism in goatpox virus p32 gene
by: ADARSH MISHRA, et al.
Published: (2020-03-01)

Genetic Evaluation of a Hereditary Familial Severe Obesity Due to Naturally Occurring Mutation in the Leptin Receptor and Structure Homology Modelling of the Mutated Molecule
by: Arash Babaei, et al.
Published: (2011-02-01)

Leptin Is Not Essential for Obesity-Associated Hypertension
by: Julia von Schnurbein, et al.
Published: (2019-07-01)

Klotho and Aminopeptidases as Early Biomarkers of Renal Injury in Zucker Obese Rats
by: Sebastián Montoro-Molina, et al.
Published: (2018-11-01)

A novel compound heterozygous leptin receptor mutation causes more severe obesity than in Leprdb/db mice
by: Claudia Berger, et al.
Published: (2021-01-01)

Treinamento físico aeróbio previne à hipertrofia cardíaca patológica e melhora a função diastólica em ratos Zucker obesos Aerobic exercise training revert pathologic cardiac hypertrophy and improves the diastolic function in obese Zucker rats
by: Diego Lopes Mendes Barretti, et al.
Published: (2011-12-01)

Apelin rs2235306 polymorphism is not related to metabolic syndrome in Egyptian women
by: Eman T. Mehanna, et al.
Published: (2015-01-01)

Characterization of IVS-110,IVS-6 and Codon 39 beta-thalassemia mutations using amplification refractory mutation system (ARMS) technique in Bisha, Saudia Arabia
by: Khaled Ismail Ghaleb
Published: (2014-08-01)

Characterization of beta-thalassemia mutations using amplification refractory mutation system (ARMS) technique in Bisha, Saudia Arabia
by: Khaled Ismail Ghaleb
Published: (2014-08-01)

A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene <i>Pi25</i>
by: Yeyang Fan, et al.
Published: (2023-09-01)

Analysis of Genetic Variation of rs4148326 Marker Located in UGT1A1 Gene Region: An Informative Marker for Molecular Diagnosis of Crigler-Najjar Syndrome
by: Zakiyeh Nadeali, et al.
Published: (2014-01-01)

Multimodal Comparison of Diabetic Neuropathy in Aged Streptozotocin-Treated Sprague–Dawley and Zucker Diabetic Fatty Rats
by: Annalisa Canta, et al.
Published: (2022-12-01)

Biochemical and Molecular Investigation of the Effect of Saponins and Terpenoids Derived from Leaves of <i>Ilex aquifolium</i> on Lipid Metabolism of Obese Zucker Rats
by: Natalia Pachura, et al.
Published: (2022-05-01)

Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits
by: Celia Raquel Quirino, et al.
Published: (2016-06-01)

Effect of Treadmill Exercise on Interleukin-15 Expression and Glucose Tolerance in Zucker Diabetic Fatty Rats
by: Hee-Jae Kim, et al.
Published: (2013-10-01)

Paraganglioma of the organ of Zuckerkandl and FH gene mutation
by: Thiti Snabboon, et al.
Published: (2020-12-01)

Studying Cytotoxic T-lymphocyte- Associated Antigen-4 (CTLA-4) gene Polymorphism in a Sample of Iraqi Women with Polycystic Ovarian Syndrome
by: Haneen Subhee Shaheed, et al.
Published: (2019-12-01)

ARMSprimer3: An open-source primer design Python program for amplification refractory mutation system PCR (ARMS-PCR)
by: Jingwen Guo, et al.
Published: (2025-04-01)

A potential adverse role for leptin and cardiac leptin receptor in the right ventricle in pulmonary arterial hypertension: effect of metformin is BMPR2 mutation-specific
by: Megha Talati, et al.
Published: (2023-10-01)

THE EFFECT SNPs R25C AND R25H LEPTIN GENE TO CHOLESTEROL AND TRIGLYCERIDE ON BLOOD OF ONGOLE CROSSBREED CATTLE
by: Nena Hilmia, et al.
Published: (2025-02-01)

Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas
by: Haider Raza, et al.
Published: (2013-12-01)

Monitoring the Expression of Leptin Receptor in Avian Model
by: Ľubica Mrázová, et al.
Published: (2023-09-01)

Phylogenetic relationships of the neon tetras Paracheirodon spp. (Characiformes: Characidae: Stethaprioninae), including comments on Petitella georgiae and Hemigrammus bleheri
by: Pedro Senna Bittencourt, et al.
Published: (2020-06-01)

GENDER-SPECIFIC INFLUENCE OF Aу MUTATION ON PROGENY METABOLIC PHENOTYPE, FETAL GROWTH AND PLACENTAL GENE EXPRESSION IN MICE
by: E. N. Makarova, et al.
Published: (2018-07-01)

The effect of combined treatment with canagliflozin and teneligliptin on glucose intolerance in Zucker diabetic fatty rats
by: Takahiro Oguma, et al.
Published: (2015-04-01)

Obese male zucker rats show basilar dendritic retraction in the medial prefrontal cortex
by: Vanessa Abigail Utrilla Ramos, et al.
Published: (2024-11-01)

Evaluation of amplification refractory mutation system (ARMS) technique for quick and accurate prenatal gene diagnosis of CHM variant in choroideremia
by: Yang L, et al.
Published: (2017-12-01)