Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of...
| Published in: | Marine Drugs |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-06-01
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| Online Access: | http://www.mdpi.com/1660-3397/16/6/191 |
| _version_ | 1852705486946697216 |
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| author | Xiao Han Lukuan Hou Jing Hou Yongyu Zhang Huayue Li Wenli Li |
| author_facet | Xiao Han Lukuan Hou Jing Hou Yongyu Zhang Huayue Li Wenli Li |
| author_sort | Xiao Han |
| collection | DOAJ |
| container_title | Marine Drugs |
| description | A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 1–5 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL. |
| format | Article |
| id | doaj-art-ec4e507350bf4f7fa8f2041cd0b3ff49 |
| institution | Directory of Open Access Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-ec4e507350bf4f7fa8f2041cd0b3ff492025-08-19T21:18:58ZengMDPI AGMarine Drugs1660-33972018-06-0116619110.3390/md16060191md16060191Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium StrainXiao Han0Lukuan Hou1Jing Hou2Yongyu Zhang3Huayue Li4Wenli Li5Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaResearch Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaA new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 1–5 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL.http://www.mdpi.com/1660-3397/16/6/191heterologous expressionrecombinantBrevibacteriummacrodiolideantibacterial |
| spellingShingle | Xiao Han Lukuan Hou Jing Hou Yongyu Zhang Huayue Li Wenli Li Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain heterologous expression recombinant Brevibacterium macrodiolide antibacterial |
| title | Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain |
| title_full | Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain |
| title_fullStr | Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain |
| title_full_unstemmed | Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain |
| title_short | Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain |
| title_sort | heterologous expression of a vioa variant activates cryptic compounds in a marine derived brevibacterium strain |
| topic | heterologous expression recombinant Brevibacterium macrodiolide antibacterial |
| url | http://www.mdpi.com/1660-3397/16/6/191 |
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