IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolution

Summary: Besides canonical microRNAs (miRNAs), sequence-based variations called isomiRs have biological relevance and diagnostic potential; however, accurate calling of these post-transcriptional modifications is challenging, especially for low input samples. Here, we present IsoSeek, a sequencing p...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Monique A.J. van Eijndhoven, Chantal Scheepbouwer, Ernesto Aparicio-Puerta, Michael Hackenberg, D. Michiel Pegtel
Format:	Article
Language:	English
Published:	Elsevier 2023-12-01
Subjects:	Bioinformatics Genetics Genomics Biotechnology and Bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723006123