A Humanized Monoclonal Antibody Cocktail to Prevent Pulmonary Ricin Intoxication

PB10 IgG<sub>1</sub>, a monoclonal antibody (MAb) directed against an immunodominant epitope on the enzymatic subunit (RTA) of ricin toxin (RT), has been shown to passively protect mice and non-human primates from an aerosolized lethal-dose RT challenge. However, it was recently demonstr...

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Bibliographic Details
Published in:Toxins
Main Authors: Yinghui Rong, Michael Pauly, Adrian Guthals, Henry Pham, Dylan Ehrbar, Larry Zeitlin, Nicholas J. Mantis
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Subjects:
toxin
lung
antibody
biodefense
prophylactic
Online Access:https://www.mdpi.com/2072-6651/12/4/215
Description
Summary:PB10 IgG<sub>1</sub>, a monoclonal antibody (MAb) directed against an immunodominant epitope on the enzymatic subunit (RTA) of ricin toxin (RT), has been shown to passively protect mice and non-human primates from an aerosolized lethal-dose RT challenge. However, it was recently demonstrated that the therapeutic efficacy of PB10 IgG1 is significantly improved when co-administered with a second MAb, SylH3, targeting RT’s binding subunit (RTB). Here we report that the PB10/SylH3 cocktail is also superior to PB10 alone when used as a pre-exposure prophylactic (PrEP) in a mouse model of intranasal RT challenge. The benefit of the PB10/SylH3 cocktail prompted us to engineer a humanized IgG1 version of SylH3 (huSylH3). The huPB10/huSylH3 cocktail proved highly efficacious in the mouse model, thereby opening the door to future testing in non-human primates.
ISSN:2072-6651

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