N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis
Emerging articles have reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6...
| Published in: | PeerJ |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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PeerJ Inc.
2023-01-01
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| Online Access: | https://peerj.com/articles/14591.pdf |
| _version_ | 1850378579848724480 |
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| author | Bao Yuan Jin Zhou |
| author_facet | Bao Yuan Jin Zhou |
| author_sort | Bao Yuan |
| collection | DOAJ |
| container_title | PeerJ |
| description | Emerging articles have reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6A reader IGF2BP1 exhibited significantly higher expression in ccRCC cells. Functionally, results by gain/loss functional assays indicated that IGF2BP1 promoted the glycolytic characteristics, including glucose uptake, lactate production and extracellular acidification rate (ECAR). Mechanistically, IGF2BP1 recognized the m6A modified sites on LDHA mRNA and enhanced its mRNA stability, thereby accelerating tumor energy metabolism. Thus, our work reveals a novel facet of the m6A that promoted mRNA stability and highlighted the functional importance of IGF2BP1 as m6A readers in post-transcriptional gene regulation. |
| format | Article |
| id | doaj-art-fb7fc7ebb7bf40e482069a92b46f68d3 |
| institution | Directory of Open Access Journals |
| issn | 2167-8359 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| spelling | doaj-art-fb7fc7ebb7bf40e482069a92b46f68d32025-08-19T22:58:23ZengPeerJ Inc.PeerJ2167-83592023-01-0111e1459110.7717/peerj.14591N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysisBao Yuan0Jin Zhou1Tianjin Beichen Traditional Chinese Medicine Hospital, Tianjin, ChinaNHC Key Laboratory of Hormones and Development, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University, Tianjin, ChinaEmerging articles have reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6A reader IGF2BP1 exhibited significantly higher expression in ccRCC cells. Functionally, results by gain/loss functional assays indicated that IGF2BP1 promoted the glycolytic characteristics, including glucose uptake, lactate production and extracellular acidification rate (ECAR). Mechanistically, IGF2BP1 recognized the m6A modified sites on LDHA mRNA and enhanced its mRNA stability, thereby accelerating tumor energy metabolism. Thus, our work reveals a novel facet of the m6A that promoted mRNA stability and highlighted the functional importance of IGF2BP1 as m6A readers in post-transcriptional gene regulation.https://peerj.com/articles/14591.pdfccRCCm6AAerobic glycolysis |
| spellingShingle | Bao Yuan Jin Zhou N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis ccRCC m6A Aerobic glycolysis |
| title | N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis |
| title_full | N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis |
| title_fullStr | N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis |
| title_full_unstemmed | N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis |
| title_short | N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis |
| title_sort | n6 methyladenosine m6a reader igf2bp1 facilitates clear cell renal cell carcinoma aerobic glycolysis |
| topic | ccRCC m6A Aerobic glycolysis |
| url | https://peerj.com/articles/14591.pdf |
| work_keys_str_mv | AT baoyuan n6methyladenosinem6areaderigf2bp1facilitatesclearcellrenalcellcarcinomaaerobicglycolysis AT jinzhou n6methyladenosinem6areaderigf2bp1facilitatesclearcellrenalcellcarcinomaaerobicglycolysis |