The effect of BSO-induced oxidative stress on histologic feature of testis: testosterone secretion and semen parameters in mice

• Published in: Iranian Journal of Basic Medical Sciences
• Main Authors: Fakhrosadat Sajjadian, Leila Roshangar, Alireza Hemmati, Mohammad Nori, Sara Soleimani-Rad, Jafar Soleimani-Rad
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2014-08-01
• Subjects: Buthionine sulfoximine Glutathione Oxidative Stress Markers Testis
• Online Access: http://ijbms.mums.ac.ir/pdf_3195_c5e0e0127d9cf67d9d26b4f671c10b53.html

Objective(s): Buthionine sulfoximine (BSO) inhibits synthesis of glutathione as the main intracellular antioxidant. The aim of the present study is to investigate the effect of BSO-induced oxidative stress on histological structure of testis, testosterone secretion and semen parameters. Materials and Methods: Thirty male BALB/c mice were divided into 3 groups. In control group, the mice did not receive any chemical. In the experimental group, the mice received 2 mmol/kg BSO for 35 days. In the sham group, the mice received the solvent of BSO (0.9% saline). After the treatment, the mice were sacrificed. Their testes were fixed in Buein’s fixative, embedded in paraffin and prepared for histological studies. To assess semen parameters, the sperms were collected from cauda epididymis. Blood samples were used for determination of super oxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH), catalase (CAT) and the serum testosterone level. The data analyzed using ANOVA and Dunnett's tests and SPSS software, version11.5. P- values at 0.05 level considered significant. Results: Data showed that in experimental group in comparison to control group; the concentration of CAT, GPX, SOD,GSH and the total level of testosterone is reduced while  MDA level is increased  significantly. The number of sperms with progressive motility were decreased               (P