Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice
Nanodiamond (ND) has recently emerged as a potential nanomaterial for nanovaccine development. Here, a plant-based haemagglutinin protein (H5.c2) of A/H5N1 virus was conjugated with detonation NDs (DND) of 3.7 nm in diameter (ND4), and high-pressure and high-temperature (HPHT) oxidative NDs of ~40–7...
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doaj-352def3c7a0544ccb9b4ca2ec7c2499e2021-07-01T00:27:37ZengMDPI AGNanomaterials2079-49912021-06-01111597159710.3390/nano11061597Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in MiceThuong Thi Ho0Van Thi Pham1Tra Thi Nguyen2Vy Thai Trinh3Tram Vi4Hsin-Hung Lin5Phuong Minh Thi Nguyen6Huyen Thi Bui7Ngoc Bich Pham8Thao Bich Thi Le9Chi Van Phan10Huan-Cheng Chang11Wesley Wei-Wen Hsiao12Ha Hoang Chu13Minh Dinh Pham14Institute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, TaiwanInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, TaiwanDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106335, TaiwanInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 100000, VietnamNanodiamond (ND) has recently emerged as a potential nanomaterial for nanovaccine development. Here, a plant-based haemagglutinin protein (H5.c2) of A/H5N1 virus was conjugated with detonation NDs (DND) of 3.7 nm in diameter (ND4), and high-pressure and high-temperature (HPHT) oxidative NDs of ~40–70 nm (ND40) and ~100–250 nm (ND100) in diameter. Our results revealed that the surface charge, but not the size of NDs, is crucial to the protein conjugation, as well as the in vitro and in vivo behaviors of H5.c2:ND conjugates. Positively charged ND4 does not effectively form stable conjugates with H5.c2, and has no impact on the immunogenicity of the protein both in vitro and in vivo. In contrast, the negatively oxidized NDs (ND40 and ND100) are excellent protein antigen carriers. When compared to free H5.c2, H5.c2:ND40, and H5.c2:ND100 conjugates are highly immunogenic with hemagglutination titers that are both 16 times higher than that of the free H5.c2 protein. Notably, H5.c2:ND40 and H5.c2:ND100 conjugates induce over 3-folds stronger production of both H5.c2-specific-IgG and neutralizing antibodies against A/H5N1 than free H5.c2 in mice. These findings support the innovative strategy of using negatively oxidized ND particles as novel antigen carriers for vaccine development, while also highlighting the importance of particle characterization before use.https://www.mdpi.com/2079-4991/11/6/1597nanodiamondsize effectsurface propertiesH5 proteinavian influenza virus A/H5N1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thuong Thi Ho Van Thi Pham Tra Thi Nguyen Vy Thai Trinh Tram Vi Hsin-Hung Lin Phuong Minh Thi Nguyen Huyen Thi Bui Ngoc Bich Pham Thao Bich Thi Le Chi Van Phan Huan-Cheng Chang Wesley Wei-Wen Hsiao Ha Hoang Chu Minh Dinh Pham |
spellingShingle |
Thuong Thi Ho Van Thi Pham Tra Thi Nguyen Vy Thai Trinh Tram Vi Hsin-Hung Lin Phuong Minh Thi Nguyen Huyen Thi Bui Ngoc Bich Pham Thao Bich Thi Le Chi Van Phan Huan-Cheng Chang Wesley Wei-Wen Hsiao Ha Hoang Chu Minh Dinh Pham Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice Nanomaterials nanodiamond size effect surface properties H5 protein avian influenza virus A/H5N1 |
author_facet |
Thuong Thi Ho Van Thi Pham Tra Thi Nguyen Vy Thai Trinh Tram Vi Hsin-Hung Lin Phuong Minh Thi Nguyen Huyen Thi Bui Ngoc Bich Pham Thao Bich Thi Le Chi Van Phan Huan-Cheng Chang Wesley Wei-Wen Hsiao Ha Hoang Chu Minh Dinh Pham |
author_sort |
Thuong Thi Ho |
title |
Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice |
title_short |
Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice |
title_full |
Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice |
title_fullStr |
Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice |
title_full_unstemmed |
Effects of Size and Surface Properties of Nanodiamonds on the Immunogenicity of Plant-Based H5 Protein of A/H5N1 Virus in Mice |
title_sort |
effects of size and surface properties of nanodiamonds on the immunogenicity of plant-based h5 protein of a/h5n1 virus in mice |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2021-06-01 |
description |
Nanodiamond (ND) has recently emerged as a potential nanomaterial for nanovaccine development. Here, a plant-based haemagglutinin protein (H5.c2) of A/H5N1 virus was conjugated with detonation NDs (DND) of 3.7 nm in diameter (ND4), and high-pressure and high-temperature (HPHT) oxidative NDs of ~40–70 nm (ND40) and ~100–250 nm (ND100) in diameter. Our results revealed that the surface charge, but not the size of NDs, is crucial to the protein conjugation, as well as the in vitro and in vivo behaviors of H5.c2:ND conjugates. Positively charged ND4 does not effectively form stable conjugates with H5.c2, and has no impact on the immunogenicity of the protein both in vitro and in vivo. In contrast, the negatively oxidized NDs (ND40 and ND100) are excellent protein antigen carriers. When compared to free H5.c2, H5.c2:ND40, and H5.c2:ND100 conjugates are highly immunogenic with hemagglutination titers that are both 16 times higher than that of the free H5.c2 protein. Notably, H5.c2:ND40 and H5.c2:ND100 conjugates induce over 3-folds stronger production of both H5.c2-specific-IgG and neutralizing antibodies against A/H5N1 than free H5.c2 in mice. These findings support the innovative strategy of using negatively oxidized ND particles as novel antigen carriers for vaccine development, while also highlighting the importance of particle characterization before use. |
topic |
nanodiamond size effect surface properties H5 protein avian influenza virus A/H5N1 |
url |
https://www.mdpi.com/2079-4991/11/6/1597 |
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