Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer
Betel quid (BQ) is a psychostimulant, an addictive substance, and a group 1 carcinogen that exhibits the potential to induce adverse health effects. Approximately, 600 million users chew a variety of BQ. Areca nut (AN) is a necessary ingredient in BQ products. Arecoline is the primary alkaloid in th...
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doaj-647840e94a6640e19e3987b1d34024892020-11-25T01:38:41ZengHindawi LimitedThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/810561810561Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral CancerPing-Ho Chen0Ka-Wo Lee1Cheng-Chieh Hsu2Jeff Yi-Fu Chen3Yan-Hsiung Wang4Ker-Kong Chen5Hui-Min David Wang6Hurng-Wern Huang7Bin Huang8School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanDepartment of Otolaryngology, Kaohsiung Medical University Hospital, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanInstitute of Biomedical Sciences, National Sun Yat-sen University, No. 70 Lienhai Road, Kaohsiung 80424, TaiwanDepartment of Biotechnology, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanDepartment of Fragrance and Cosmetic Science, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanInstitute of Biomedical Sciences, National Sun Yat-sen University, No. 70 Lienhai Road, Kaohsiung 80424, TaiwanDepartment of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanBetel quid (BQ) is a psychostimulant, an addictive substance, and a group 1 carcinogen that exhibits the potential to induce adverse health effects. Approximately, 600 million users chew a variety of BQ. Areca nut (AN) is a necessary ingredient in BQ products. Arecoline is the primary alkaloid in the AN and can be metabolized through the cytochrome P450 (CYP) superfamily by inducing reactive oxygen species (ROS) production. Full-length CYP26B1 is related to the development of oral pharyngeal cancers. We investigated whether a splice variant of CYP26B1 is associated with the occurrence of ROS related oral and pharyngeal cancer. Cytotoxicity assays were used to measure the effects of arecoline on cell viability in a dose-dependent manner. In vitro and in vivo studies were conducted to evaluate the expression of the CYP26B1 splice variant. The CYP26B1 splice variant exhibited lower expression than did full-length CYP26B1 in the human gingival fibroblast-1 and Ca9-22 cell models. Increased expression of the CYP26B1 splice variant was observed in human oral cancer tissue compared with adjacent normal tissue, and increased expression was observed in patients at a late tumor stage. Our results suggested that the CYP26B1 splice variant is associated with the occurrence of BQ-related oral cancer.http://dx.doi.org/10.1155/2014/810561 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ping-Ho Chen Ka-Wo Lee Cheng-Chieh Hsu Jeff Yi-Fu Chen Yan-Hsiung Wang Ker-Kong Chen Hui-Min David Wang Hurng-Wern Huang Bin Huang |
spellingShingle |
Ping-Ho Chen Ka-Wo Lee Cheng-Chieh Hsu Jeff Yi-Fu Chen Yan-Hsiung Wang Ker-Kong Chen Hui-Min David Wang Hurng-Wern Huang Bin Huang Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer The Scientific World Journal |
author_facet |
Ping-Ho Chen Ka-Wo Lee Cheng-Chieh Hsu Jeff Yi-Fu Chen Yan-Hsiung Wang Ker-Kong Chen Hui-Min David Wang Hurng-Wern Huang Bin Huang |
author_sort |
Ping-Ho Chen |
title |
Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer |
title_short |
Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer |
title_full |
Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer |
title_fullStr |
Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer |
title_full_unstemmed |
Expression of a Splice Variant of CYP26B1 in Betel Quid-Related Oral Cancer |
title_sort |
expression of a splice variant of cyp26b1 in betel quid-related oral cancer |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
2356-6140 1537-744X |
publishDate |
2014-01-01 |
description |
Betel quid (BQ) is a psychostimulant, an addictive substance, and a group 1 carcinogen that exhibits the potential to induce adverse health effects. Approximately, 600 million users chew a variety of BQ. Areca nut (AN) is a necessary ingredient in BQ products. Arecoline is the primary alkaloid in the AN and can be metabolized through the cytochrome P450 (CYP) superfamily by inducing reactive oxygen species (ROS) production. Full-length CYP26B1 is related to the development of oral pharyngeal cancers. We investigated whether a splice variant of CYP26B1 is associated with the occurrence of ROS related oral and pharyngeal cancer. Cytotoxicity assays were used to measure the effects of arecoline on cell viability in a dose-dependent manner. In vitro and in vivo studies were conducted to evaluate the expression of the CYP26B1 splice variant. The CYP26B1 splice variant exhibited lower expression than did full-length CYP26B1 in the human gingival fibroblast-1 and Ca9-22 cell models. Increased expression of the CYP26B1 splice variant was observed in human oral cancer tissue compared with adjacent normal tissue, and increased expression was observed in patients at a late tumor stage. Our results suggested that the CYP26B1 splice variant is associated with the occurrence of BQ-related oral cancer. |
url |
http://dx.doi.org/10.1155/2014/810561 |
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