Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.

Bibliographic Details
Main Authors: Jasmine M. Hershewe, Katherine F. Warfel, Shaelyn M. Iyer, Justin A. Peruzzi, Claretta J. Sullivan, Eric W. Roth, Matthew P. DeLisa, Neha P. Kamat, Michael C. Jewett
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-22329-3