CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

Raffinose family oligosaccharides (RFOs) are major soluble carbohydrates in soybean seeds that cannot be digested by human and other monogastric animals. Hence, a major goal is to reduce RFO levels to improve the nutritional quality of soybean. In this study, we utilized a dual gRNAs CRISPR/Cas9 sys...

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Bibliographic Details
Main Authors: Huy Le, Nhung Hong Nguyen, Dong Thị Ta, Thao Nhu Thi Le, Thao Phuong Bui, Ngoc Thu Le, Cuong Xuan Nguyen, Hardy Rolletschek, Gary Stacey, Minviluz G. Stacey, Ngoc Bich Pham, Phat Tien Do, Ha Hoang Chu
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
GmGOLS1A
GmGOLS1B
raffinose family oligosaccharides
soybean
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2020.612942/full

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https://www.frontiersin.org/articles/10.3389/fpls.2020.612942/full

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