A comparison and optimization of methods and factors affecting the transformation of Escherichia coli
DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2–CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Portland Press, Biochemical Society
2013-12-01
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| Series: | Bioscience Reports |
| Subjects: | |
| Online Access: | http://www.bioscirep.org/bsr/033/e086/bsr033e086.htm |