Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence
Schistosomiasis japonica is a water-borne trematode infection transmitted by different subspecies of Oncomelania hupensis. As parasites may either co-evolve or locally adapt with their hosts, snail diversity, as revealed by morphometric and genetic studies, may reflect parasite diversity and elucida...
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doaj-e4c52962da0a4fb19154b048069914d92020-11-24T23:20:39ZengUniversity of the PhilippinesScience Diliman0115-78092012-08182017-12-012923250Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA SequenceJames Christopher C. Chua0Ian Kim B. Tabios1Pebbles Grayle P. Tamayo2Lydia R. Leonardo3Ian Kendrich C. Fontanilla4Raffy Jay C. Fornillos5Emmanuel Ryan C. De Chavez6Takeshi Agatsuma7Mihoko Kikuchi8Naoko Kato-Hayashi9 Yuichi Chigusa10University of the PhilippinesUniversity of the PhilippinesUniversity of the PhilippinesUniversity of the PhilippinesUniversity of the PhilippinesUniversity of the PhilippinesUniversity of the PhilippinesKochi Medical SchoolNagasaki UniversityDokkyo Medical UniversityDokkyo Medical UniversitySchistosomiasis japonica is a water-borne trematode infection transmitted by different subspecies of Oncomelania hupensis. As parasites may either co-evolve or locally adapt with their hosts, snail diversity, as revealed by morphometric and genetic studies, may reflect parasite diversity and elucidate snail susceptibility and transmission patterns. This study aimed to compare isolates of O. h. quadrasi based on a 342-bp fragment of the 16S ribosomal RNA gene. O. h. quadrasi isolates were collected from nine provinces known to have S. japonicum in the Philippines, namely Cagayan Valley, Bohol, Negros Occidental, Leyte, Davao, Davao del Sur, Mindoro Oriental, Northern Samar, and Sorsogon. O. h. hupensis and O. h. nosophora isolates were also collected from China and Japan, respectively. The 16S ribosomal RNA gene of each specimen was amplified and sequenced. Phylogenetic and network analyses based on the 221 16S rRNA gene sequences revealed that O. h. quadrasi clustered as a distinct clade from the two other subspecies. Of the four identified haplotypes for O. h. quadrasi, two haplotypes were from Negros Oriental (Ohq2 and Ohq3), and one haplotype was from Bohol (Ohq4). The isolates from the remaining seven provinces shared a common haplotype (Ohq1). The current study was able to show the relationship among O. hupensis subspecies and demonstrate the limited ability of mitochondrial 16S ribosomal molecular marker in differentiating O. h. quadrasi geographic strains in the Philippines. http://www.journals.upd.edu.ph/index.php/sciencediliman/article/view/5805Oncomelania hupensis quadrasiSchistosoma japonicumSchistosomiasis japonicaSnail Intermediate HostHaplotype16S Ribosomal RNA Gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
James Christopher C. Chua Ian Kim B. Tabios Pebbles Grayle P. Tamayo Lydia R. Leonardo Ian Kendrich C. Fontanilla Raffy Jay C. Fornillos Emmanuel Ryan C. De Chavez Takeshi Agatsuma Mihoko Kikuchi Naoko Kato-Hayashi Yuichi Chigusa |
spellingShingle |
James Christopher C. Chua Ian Kim B. Tabios Pebbles Grayle P. Tamayo Lydia R. Leonardo Ian Kendrich C. Fontanilla Raffy Jay C. Fornillos Emmanuel Ryan C. De Chavez Takeshi Agatsuma Mihoko Kikuchi Naoko Kato-Hayashi Yuichi Chigusa Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence Science Diliman Oncomelania hupensis quadrasi Schistosoma japonicum Schistosomiasis japonica Snail Intermediate Host Haplotype 16S Ribosomal RNA Gene |
author_facet |
James Christopher C. Chua Ian Kim B. Tabios Pebbles Grayle P. Tamayo Lydia R. Leonardo Ian Kendrich C. Fontanilla Raffy Jay C. Fornillos Emmanuel Ryan C. De Chavez Takeshi Agatsuma Mihoko Kikuchi Naoko Kato-Hayashi Yuichi Chigusa |
author_sort |
James Christopher C. Chua |
title |
Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence |
title_short |
Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence |
title_full |
Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence |
title_fullStr |
Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence |
title_full_unstemmed |
Genetic Comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the Intermediate Host of Schistosoma japonicum in the Philippines, Based on 16S Ribosomal RNA Sequence |
title_sort |
genetic comparison of oncomelania hupensis quadrasi (möllendorf, 1895) (gastropoda: pomatiopsidae), the intermediate host of schistosoma japonicum in the philippines, based on 16s ribosomal rna sequence |
publisher |
University of the Philippines |
series |
Science Diliman |
issn |
0115-7809 2012-0818 |
publishDate |
2017-12-01 |
description |
Schistosomiasis japonica is a water-borne trematode infection transmitted by different subspecies of Oncomelania hupensis. As parasites may either co-evolve or locally adapt with their hosts, snail diversity, as revealed by morphometric and genetic studies, may reflect parasite diversity and elucidate snail susceptibility and transmission patterns. This study aimed to compare isolates of O. h. quadrasi based on a 342-bp fragment of the 16S ribosomal RNA gene. O. h. quadrasi isolates were collected from nine provinces known to have S. japonicum in the Philippines, namely Cagayan Valley, Bohol, Negros Occidental, Leyte, Davao, Davao del Sur, Mindoro Oriental, Northern Samar, and Sorsogon. O. h. hupensis and O. h. nosophora isolates were also collected from China and Japan, respectively. The 16S ribosomal RNA gene of each specimen was amplified and sequenced. Phylogenetic and network analyses based on the 221 16S rRNA gene sequences revealed that O. h. quadrasi clustered as a distinct clade from the two other subspecies. Of the four identified haplotypes for O. h. quadrasi, two haplotypes were from Negros Oriental (Ohq2 and Ohq3), and one haplotype was from Bohol (Ohq4). The isolates from the remaining seven provinces shared a common haplotype (Ohq1). The current study was able to show the relationship among O. hupensis subspecies and demonstrate the limited ability of mitochondrial 16S ribosomal molecular marker in differentiating O. h. quadrasi geographic strains in the Philippines.
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topic |
Oncomelania hupensis quadrasi Schistosoma japonicum Schistosomiasis japonica Snail Intermediate Host Haplotype 16S Ribosomal RNA Gene |
url |
http://www.journals.upd.edu.ph/index.php/sciencediliman/article/view/5805 |
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