Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

Abstract CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we...

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Bibliographic Details
Main Authors: Fei Teng, Jing Li, Tongtong Cui, Kai Xu, Lu Guo, Qingqin Gao, Guihai Feng, Chuanyuan Chen, Dali Han, Qi Zhou, Wei Li
Format: Article
Language:English
Published: BMC 2019-02-01
Series:Genome Biology
Subjects:
CRISPR-Cas12a/Cpf1
crRNA
Genome editing
Human cells
Mouse cells
Online Access:http://link.springer.com/article/10.1186/s13059-019-1620-8

Internet

http://link.springer.com/article/10.1186/s13059-019-1620-8

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