Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons

Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons

Bibliographic Details
Main Author: Mantha, Madhavi
Language:English
Published: Wright State University / OhioLINK 2008
Subjects:
Chemistry
Polycyclic Aromatic Hydro Carbons
PAHs
Polystyrene-divinylbenzene
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=wright1213730320
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-wright12137303202021-08-03T06:16:44Z Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons Mantha, Madhavi Chemistry Polycyclic Aromatic Hydro Carbons PAHs Polystyrene-divinylbenzene The purpose of the research was to design a method using high-performance liquid chromatography (HPLC) that would separate the sixteen polycyclic aromatic hydrocarbons (PAHs) designated by Environmental Protection Agency (EPA) as priority pollutants on two different kinds of polystyrene (PS) in the reversed phase. One was a zirconia-based column coated with polystyrene. The other was a polystyrene divinylbenzene (PS-DVB) column. The idea behind using these columns was to take advantage of extreme retention of PAHs by these columns by using high temperatures or temperature programming in order to reduce the amount of organic modifier in the mobile phases to perform analytical separations and achieve faster LC separations. Separation of the sixteen PAHs was attempted using PS-coated zirconia and the optimum conditions consisted of thermal programming as well as mobile phase gradient by running the sample at 60°C for the first 15 minutes and then at 50°C for the next fifteen minutes and using 30% acetonitrile (ACN) from 0-10 minutes, 30-50% ACN from 10-15 minutes and 50-75% ACN from 15-30 minutes. Co-elution of geometric isomers, benz(a)anthracene with chrysene and benzo(b)fluoranthene with benzo(k)fluoranthene was observed. The optimum conditions using the PS-DVB column were temperature 70°C isothermal for 5 min, 70-110°C gradient at 5.33°C min<sup>-1</sup> for the next 7.5 min and 110°C isothermal for the next 17.5 min, mobile phase 50-90% ACN, remaining H<sub>2</sub>O:MeOH 1:1 (v/v), flow rate 2 mL min<sup>-1</sup>. Again co-elution of geometric isomers, benz(a)anthracene with chrysene and benzo(b)fluoranthene with benzo(k)fluoranthene was observed. The last three anlaytes, dibenz(a,h)anthracene, indeno(1,2,3,cd)pyrene and benzo(ghi)perylene had better separation with one peak and two shoulders, but could not be resolved completely. No combination of mobile phases containing THF, ACN, H2O and/or MeOH and no temperature program was able to completely resolve the EPA 16 PAHs better than typical silica-based C-18 columns. The PS columns did, however show potential for separation of PAHs and their environmental derivatives with two to three rings, such as those found in the vapor phase in the environment. 2008-07-16 English text Wright State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=wright1213730320 http://rave.ohiolink.edu/etdc/view?acc_num=wright1213730320 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Chemistry
Polycyclic Aromatic Hydro Carbons
PAHs
Polystyrene-divinylbenzene
spellingShingle Chemistry
Polycyclic Aromatic Hydro Carbons
PAHs
Polystyrene-divinylbenzene
Mantha, Madhavi
Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
author Mantha, Madhavi
author_facet Mantha, Madhavi
author_sort Mantha, Madhavi
title Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
title_short Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
title_full Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
title_fullStr Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
title_full_unstemmed Polystyrene as a Medium in Reverse-Phase Separation Of Polycyclic Aromatic Hydrocarbons
title_sort polystyrene as a medium in reverse-phase separation of polycyclic aromatic hydrocarbons
publisher Wright State University / OhioLINK
publishDate 2008
url http://rave.ohiolink.edu/etdc/view?acc_num=wright1213730320
work_keys_str_mv AT manthamadhavi polystyreneasamediuminreversephaseseparationofpolycyclicaromatichydrocarbons
_version_ 1719433961277489152

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