Fit-for-purpose based testing and validation of antibodies to amino- and carboxy-terminal domains of cannabinoid receptor 1

Fit-for-purpose based testing and validation of antibodies to amino- and carboxy-terminal domains of cannabinoid receptor 1

Specific and selective anti-CB1 antibodies are among the most powerful research tools to unravel the complex biological processes mediated by the CB1 receptor in both physiological and pathological conditions. However, low performance of antibodies remains a major source of inconsistency between res...

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Bibliographic Details
Main Authors: Aretxabala, X. (Author), Barrondo, S. (Author), Borrega-Román, L. (Author), Echeazarra, L. (Author), García del Caño, G. (Author), Goicolea, M.A (Author), González-Burguera, I. (Author), Ledent, C. (Author), López de Jesús, M. (Author), Mato, S. (Author), Matute, C. (Author), Sallés, J. (Author), Saumell-Esnaola, M. (Author)
Format: Article
Language:English
Published: Springer Science and Business Media Deutschland GmbH 2021
Subjects:
adult
amino terminal sequence
Amino-terminus
animal
animal experiment
animal tissue
Animals
Antibodies
antibody
antibody specificity
Antibody specificity
antigen retrieval
Antigen retrieval
Article
bovine serum albumin
brain cortex
brain membrane
cannabinoid 1 receptor
carboxy terminal sequence
Carboxy-terminus
CB1 receptor
CB1-knockout mice
cell fractionation
cell nucleus
controlled study
extracellular matrix
HEK293 cell line
human
human cell
immunoblotting
immunofluorescence
immunofluorescence assay
immunohistochemistry
immunology
immunoreactivity
knockout mouse
male
mathematical model
Mice
Mice, Knockout
mouse
nonhuman
rat
Rats
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1
scanning electron microscopy
signal noise ratio
Sprague Dawley rat
surface property
tissue fixation
tissue section
Western blotting
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