Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures

Summary: Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that en...

Full description

Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Satoshi Konishi, Aleksandra Tata, Purushothama Rao Tata
Format:	Article
Language:	English
Published:	Elsevier 2022-06-01
Subjects:	Cell Biology Cell culture Cell isolation Stem Cells Organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722003276