Positive Selection of Specific Antibodies Produced against Fusion Proteins
A method for the positive selection of specific antibodies for target proteins expressed as fusion proteins for the production of antiserum is presented. As proof of concept, the fusion protein FLAG::His::GFP::His::FLAG was expressed in <i>Escherichia coli</i>, purified, and used for the...
| Published in: | Methods and Protocols |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
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| Subjects: | |
| Online Access: | https://www.mdpi.com/2409-9279/3/2/37 |