Protocol for in vivo elimination of avian auditory hair cells, multiplexed mRNA detection, immunohistochemistry, and S-phase labeling

Summary: The avian inner ear can naturally regenerate sensory hair cells and is therefore an ideal candidate for investigating mechanisms leading to hair cell regeneration and functional recovery. Here, we present a surgical protocol for eliminating auditory hair cells via sisomicin injection into t...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Mitsuo P. Sato, Austin P. Huang, Stefan Heller, Nesrine Benkafadar
Format:	Article
Language:	English
Published:	Elsevier 2024-06-01
Subjects:	model organisms antibody in situ hybridization
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724002831