Development of Dry and Liquid Duplex Reagent Mix-Based Polymerase Chain Reaction Assays as Novel Tools for the Rapid and Easy Quantification of Bovine Leukemia Virus (BLV) Proviral Loads

Bovine leukemia virus (BLV) is prevalent worldwide, causing serious problems in the cattle industry. The BLV proviral load (PVL) is a useful index for estimating disease progression and transmission risk. We previously developed a quantitative real-time PCR (qPCR) assay to measure the PVL using the...

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Bibliographic Details

Published in:	Viruses
Main Authors:	Sonoko Watanuki, Kazuyuki Shoji, Masaki Izawa, Mitsuaki Okami, Yingbao Ye, Aronggaowa Bao, Yulin Liu, Etsuko Saitou, Kimikazu Sugiyama, Michiru Endo, Yasunobu Matsumoto, Yoko Aida
Format:	Article
Language:	English
Published:	MDPI AG 2024-06-01
Subjects:	bovine leukemia virus (BLV) proviral load (PVL) quantitative real-time PCR (qPCR) duplex singleplex liquid
Online Access:	https://www.mdpi.com/1999-4915/16/7/1016