Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway

Metabolic engineering strategies have been successfully implemented to improve the production of isobutanol, a next-generation biofuel, in Saccharomyces cerevisiae. Here, we explore how two of these strategies, pathway re-localization and redox cofactor-balancing, affect the performance and physiolo...

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Bibliographic Details

Published in:	Synthetic and Systems Biotechnology
Main Authors:	Francesca V. Gambacorta, Ellen R. Wagner, Tyler B. Jacobson, Mary Tremaine, Laura K. Muehlbauer, Mick A. McGee, Justin J. Baerwald, Russell L. Wrobel, John F. Wolters, Mike Place, Joshua J. Dietrich, Dan Xie, Jose Serate, Shabda Gajbhiye, Lisa Liu, Maikayeng Vang-Smith, Joshua J. Coon, Yaoping Zhang, Audrey P. Gasch, Daniel Amador-Noguez, Chris Todd Hittinger, Trey K. Sato, Brian F. Pfleger
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2022-06-01
Subjects:	Saccharomyces cerevisiae Isobutanol Functional genomics analysis Pathway localization Fe incorporation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2405805X2200014X