Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics

Summary: Lamellipodia are sheet-like protrusions essential for cell migration and endocytosis, but their ultrastructural dynamics remain poorly understood because conventional electron microscopy lacks temporal resolution. Here, we combined optogenetics with cryo-electron tomography (cryo-ET) to vis...

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Bibliographic Details

Published in:	iScience
Main Authors:	Hironori Inaba, Tsuyoshi Imasaki, Kazuhiro Aoyama, Shogo Yoshihara, Hiroko Takazaki, Takayuki Kato, Hidemasa Goto, Kaoru Mitsuoka, Ryo Nitta, Takao Nakata
Format:	Article
Language:	English
Published:	Elsevier 2025-06-01
Subjects:	Cell biology Organizational aspects of cell biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004225007904