Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9

Herpes simplex virus (HSV) establishes lifelong latent infection and can cause serious human disease, but current antiviral therapies target lytic but not latent infection. We screened for sgRNAs that cleave HSV-1 DNA sequences efficiently in vitro and used these sgRNAs to observe the first editing...

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Bibliographic Details

Published in:	eLife
Main Authors:	Hyung Suk Oh, Werner M Neuhausser, Pierce Eggan, Magdalena Angelova, Rory Kirchner, Kevin C Eggan, David M Knipe
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2019-12-01
Subjects:	Genome editing CRISPR herpes simplex virus lytic replication latent infection reactivation
Online Access:	https://elifesciences.org/articles/51662