Design and validation of a semi-quantitative microneutralization assay for human Metapneumovirus A1 and B1 subtypes

Abstract Since 2001, human Metapneumovirus has been a significant cause of human respiratory disease worldwide, and no vaccine or preventive treatment is currently available. The ELISA-based live virus microneutralization assay is a method to detect neutralizing antibodies against a target pathogen....

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Giulia Riolo, Valentina Biagini, Noemi Guerrini, Giulia Roscia, Roberta Antonelli, Ginevra Giglioli, Maria Alfreda Stincarelli, Pietro Piu, Carolina Bonifazi, Simona De Grazia, Mariangela Pizzo, Piero Lovreglio, Angela Stufano, Claudia Maria Trombetta, Alessandro Manenti, Emanuele Montomoli, Francesca Dapporto
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-04-01
Online Access:	https://doi.org/10.1038/s41598-025-96567-6