Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches

DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications. In vitro, DNA polymerases are used for DNA manipulations, including cloning, PCR, site-directed mutagenesis, sequencing, and others. Understanding th...

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Bibliographic Details

Published in:	Bioengineering
Main Authors:	Aleksandra A. Kuznetsova, Nikita A. Kuznetsov
Format:	Article
Language:	English
Published:	MDPI AG 2023-09-01
Subjects:	PCR family B DNA polymerase fusion DNA polymerase mutagenesis
Online Access:	https://www.mdpi.com/2306-5354/10/10/1150