DNA PCR Assays for Igh Rearrangement

This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].

Bibliographic Details
Published in:Bio-Protocol
Main Authors: Yusuke Satoh, Takao Sudo, Takafumi Yokota
Format: Article
Language:English
Published: Bio-protocol LLC 2014-02-01
Online Access:https://bio-protocol.org/en/bpdetail?id=1046&type=0