Establishment of a dual droplet digital PCR method for detecting the Brucella abortus A19-ΔVirB12 strains

Brucellosis is a zoonosis that occurs worldwide, and vaccination is the main strategy for controlling it. In China, the Brucella abortus A19-ΔVirB12 strain is utilized in main vaccines. However, a high-sensitivity nucleic acid detection method to effectively differentiate Brucella infections from im...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Ruixue Xue, Zunfeng Chu, Linlin Xing, Zixin Jiang, Wenduo Jiang, Yingli Shang, Fangkun Wang, Hongmei Wang, Yuyu Zhang, Yanling Wang, Yu Miao, Xinglin Zhang, Mingjun Sun, Zouran Lan, Yue Zhang
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-10-01
Subjects:	brucellosis A19-ΔVirB12 strain VirB8 gene VirB12 gene ddPCR
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1684156/full