Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Abstract Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food. These methods rely on the detection of gene sequences to indicate the presence of viable bacteria. This...

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发表在:	BMC Microbiology
Main Authors:	Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber
格式:	文件
语言:	英语
出版:	BMC 2022-09-01
主题:	16S rRNA amplicon sequencing Bacterial culture Culture independent detection Biotyper DNA extraction Metagenomics
在线阅读:	https://doi.org/10.1186/s12866-022-02650-7