Calcium-Free and Cytochalasin B Treatment Inhibits Blastomere Fusion in 2-Cell Stage Embryos for the Generation of Floxed Mice via Sequential Electroporation

The generation of conditional knockout mice using the Cre-loxP system is advantageous for the functional analysis of genes. Flanked by two loxP sites (floxed) mice can be directly obtained from fertilized eggs by the CRISPR/Cas9 genome editing system. We previously reported that sequential knock-in...

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Bibliographic Details

Published in:	Cells
Main Authors:	Takuro Horii, Ryosuke Kobayashi, Mika Kimura, Sumiyo Morita, Izuho Hatada
Format:	Article
Language:	English
Published:	MDPI AG 2020-04-01
Subjects:	CRISPR/Cas9 knock-in flox electroporation 2-cell fusion
Online Access:	https://www.mdpi.com/2073-4409/9/5/1088