Optimized metrics for orthogonal combinatorial CRISPR screens

Abstract CRISPR-based gene perturbation enables unbiased investigations of single and combinatorial genotype-to-phenotype associations. In light of efforts to map combinatorial gene dependencies at scale, choosing an efficient and robust CRISPR-associated (Cas) nuclease is of utmost importance. Even...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, Simone Schaubeck, Chase L. Beisel, Manuel Kaulich
Format:	Article
Language:	English
Published:	Nature Portfolio 2023-05-01
Online Access:	https://doi.org/10.1038/s41598-023-34597-8