An automated high throughput solution for DNA extraction and bisulfite-conversion from high volume liquid biopsy specimens: sample preparation for epigenetic analysis

Abstract Objective DNA methylation analysis via real-time PCR or other analytical techniques requires purified bisulfite converted DNA. We report on an automated high throughput solution for DNA extraction, bisulfite-conversion, and purification of 96 samples with an input volume of up to 3.5 mL of...

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Bibliographic Details

Published in:	BMC Research Notes
Main Authors:	Sebastian Rausch, Oliver Hasinger, Thomas König, Anne Schlegel, Gunter Weiss
Format:	Article
Language:	English
Published:	BMC 2019-08-01
Subjects:	Liquid biopsy Cell free DNA cfDNA High throughput DNA extraction Bisulfite conversion DNA methylation
Online Access:	http://link.springer.com/article/10.1186/s13104-019-4595-3