Selection of reference genes in Thermothelomyces fergusii: insights from genomic and expression analysis in response to different carbon sources

Abstract Background RNA expression analysis using reverse transcription qPCR requires reference genes for accurate data normalization. However, some reference genes can show significant variability in specific biological samples, raising concerns about the reliability of this method. This study asse...

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发表在:	BMC Genomics
Main Authors:	Abdul Waheed, Yi Chen, Hafiz Muhammad Rizwan, Muhammad Adnan, Xuekun Ma, Gang Liu
格式:	文件
语言:	英语
出版:	BMC 2025-07-01
主题:	Thermothelomyces Fergusii RefFinder Sugar treatments Data normalization Fermentation medium
在线阅读:	https://doi.org/10.1186/s12864-025-11772-x