Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives

Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest alo...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	John LaCava, Kelly R. Molloy, Martin S. Taylor, Michal Domanski, Brian T. Chait, Michael P. Rout
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2015-03-01
Subjects:	protein complex protein purification affinity proteomics interactomics
Online Access:	https://www.future-science.com/doi/10.2144/000114262