Protocol for isolating and characterizing human vitreous immune cell infiltrates by flow cytometry and single-cell transcriptomic studies

Summary: Vitreous infiltrating immune cells represent an accessible tissue sample that can provide valuable information on the intraocular immune response without compromising the structural and functional integrity of the eye. Here, we present our customized protocol for isolating and characterizin...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Sandhya Rani Bhanja, Sayantan Ghosh, Pankaj Kumar, Tulasi Nagabandi, Arun Raina, Kaiser Alam, John V. Forrester, Soumyava Basu
Format:	Article
Language:	English
Published:	Elsevier 2025-06-01
Subjects:	Cell Biology Cell isolation Single Cell Flow Cytometry Health Sciences Genomics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725002369