Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Abstract Site-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA editing without generating toxic DNA dou...

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Bibliographic Details

Published in:	Nature Communications
Main Authors:	Charlotte Cautereels, Jolien Smets, Jonas De Saeger, Lloyd Cool, Yanmei Zhu, Anna Zimmermann, Jan Steensels, Anton Gorkovskiy, Thomas B. Jacobs, Kevin J. Verstrepen
Format:	Article
Language:	English
Published:	Nature Portfolio 2024-02-01
Online Access:	https://doi.org/10.1038/s41467-024-44996-8