Chromatographic single-step purification of tagless proteins using gp41-1 split inteins

The current trend in biopharmaceutical drug manufacturing is towards increasing potency and complexity of products such as peptide scaffolds, oligonucleotides and many more. Therefore, a universal affinity purification step is important in order to meet the requirements for cost and time efficient d...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Michael Knapp, Vanessa Kohl, Tatjana Best, Oliver Rammo, Sybille Ebert
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-01-01
Subjects:	gp41-1 split intein tagless purification affinity chromatography cleaning
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1319916/full