Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures

Summary: Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Anxo Martinez-Ordoñez, Tania Cid-Diaz, Angeles Duran, Qixiu Han, Jorge Moscat, Maria T. Diaz-Meco
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Cell Biology Cell Culture Cancer Microscopy Molecular Biology Cell Differentiation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002010