Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B

Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro selection or directed evolution on existing or d...

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Bibliographic Details

Published in:	Results in Chemistry
Main Authors:	Kun-Hwa Lee, Mark D. Distefano, Burckhard Seelig
Format:	Article
Language:	English
Published:	Elsevier 2023-12-01
Subjects:	Pyridoxal 5′-phosphate (PLP) Cofactor immobilization Bioconjugation NHS ester chemistry p-Diazobenzoyl derivatized resin
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211715623002837