Protocol for the generation, characterization, and functional assays of organoid cultures from normal and cancer-prone human esophageal tissues

Summary: Multiple sampling strategies to cover different or dynamic stages of malignant continuum with organoid cultures provide a valuable platform for epithelium homeostasis, transformation, and cancer progression. Here, we present a protocol to initiate, culture, passage, and characterize organoi...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Shaosen Zhang, Guoyu Cheng, Shihao Zhu, Dongxin Lin, Chen Wu
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	Cell Biology Cell culture Cancer Organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724004817