Protocol for the generation, characterization, and functional assays of organoid cultures from normal and cancer-prone human esophageal tissues

• الحاوية / القاعدة: STAR Protocols
• المؤلفون الرئيسيون: Shaosen Zhang, Guoyu Cheng, Shihao Zhu, Dongxin Lin, Chen Wu
• التنسيق: مقال
• اللغة: الإنجليزية
• منشور في: Elsevier 2024-12-01
• الموضوعات: Cell Biology; Cell culture; Cancer; Organoids
• الوصول للمادة أونلاين: http://www.sciencedirect.com/science/article/pii/S2666166724004817
• تدمد: 2666-1667

Summary: Multiple sampling strategies to cover different or dynamic stages of malignant continuum with organoid cultures provide a valuable platform for epithelium homeostasis, transformation, and cancer progression. Here, we present a protocol to initiate, culture, passage, and characterize organoids from normal and cancer-prone human esophageal tissues. We describe steps for multiple sampling of malignant continuum and the initiation and maintenance of multi-stage organoids. We then detail procedures for the histological characterization of organoids and co-culture systems based on organoids and stromal cells.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.