A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli

Itaconic acid (ITA), an effective alternative fossil fuel, derives from the bypass pathway of the tricarboxylic acid (TCA) cycle. Therefore, the imbalance of metabolic flux between TCA cycle and ITA biosynthetic pathway seriously limits the production of ITA. The optimization of flux distribution be...

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Bibliographic Details

Published in:	Synthetic and Systems Biotechnology
Main Authors:	Ming Zhao, Yuting Li, Fengqing Wang, Yuhong Ren, Dongzhi Wei
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2022-09-01
Subjects:	CRISPRi Dynamic regulation Itaconic acid Biosensor
Online Access:	http://www.sciencedirect.com/science/article/pii/S2405805X22000552