A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster

The ability to clone large DNA fragments from genomes is valuable for both basic and applied research, such as the construction of synthetic genomes, and the expression of biosynthetic gene clusters (BGCs) for natural product discovery. Here, we report a fast and efficient platform for the direct ca...

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Bibliographic Details

Published in:	Microorganisms
Main Authors:	Yujing Guo, Guang Cai, Huiying Li, Zhenquan Lin, Shuobo Shi, Jin Jin, Zihe Liu
Format:	Article
Language:	English
Published:	MDPI AG 2024-07-01
Subjects:	CRISPR/Cas9 <i>in vitro</i> DNA assembly synthetic genomes biosynthetic gene cluster
Online Access:	https://www.mdpi.com/2076-2607/12/7/1462