A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster

The ability to clone large DNA fragments from genomes is valuable for both basic and applied research, such as the construction of synthetic genomes, and the expression of biosynthetic gene clusters (BGCs) for natural product discovery. Here, we report a fast and efficient platform for the direct ca...

وصف كامل

التفاصيل البيبلوغرافية
الحاوية / القاعدة:Microorganisms
المؤلفون الرئيسيون: Yujing Guo, Guang Cai, Huiying Li, Zhenquan Lin, Shuobo Shi, Jin Jin, Zihe Liu
التنسيق: مقال
اللغة:الإنجليزية
منشور في: MDPI AG 2024-07-01
الموضوعات:
CRISPR/Cas9
<i>in vitro</i> DNA assembly
synthetic genomes
biosynthetic gene cluster
الوصول للمادة أونلاين:https://www.mdpi.com/2076-2607/12/7/1462

الانترنت

https://www.mdpi.com/2076-2607/12/7/1462

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