A multiplex primer design algorithm for target amplification of continuous genomic regions

Abstract Background Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more...

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Bibliographic Details

Published in:	BMC Bioinformatics
Main Authors:	Ahmet Rasit Ozturk, Tolga Can
Format:	Article
Language:	English
Published:	BMC 2017-06-01
Subjects:	Next Generation sequencing Target amplification Multiplex PCR Primer design
Online Access:	http://link.springer.com/article/10.1186/s12859-017-1716-7