Thermodynamics shapes the in vivo enzyme burden of glycolytic pathways

ABSTRACT Thermodynamically constrained reactions and pathways are hypothesized to impose greater protein demands on cells, requiring higher enzyme amounts to sustain a given flux compared to those with stronger thermodynamics. To test this, we quantified the absolute concentrations of glycolytic enz...

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Bibliographic Details
Published in:mBio
Main Authors: Daven B. Khana, Annie Jen, Evgenia Shishkova, Kirsten Fisher, Eashant Thusoo, Jonathan Williams, Alex Henkel, David M. Stevenson, Joshua J. Coon, Daniel Amador-Noguez
Format: Article
Language:English
Published: American Society for Microbiology 2025-10-01
Subjects:
thermodynamics
glycolysis
proteomics
metabolic flux
enzyme burden
Zymomonas mobilis
Online Access:https://journals.asm.org/doi/10.1128/mbio.01837-25

Internet

https://journals.asm.org/doi/10.1128/mbio.01837-25

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