Investigating fungal diversity through metabarcoding for environmental samples: assessment of ITS1 and ITS2 Illumina sequencing using multiple defined mock communities with different classification methods and reference databases

Abstract An important challenge in taxonomic classification of environmental samples is capturing the real diversity by identifying all species present in a sample. Metabarcoding approaches are often employed to identify species in complex samples. The internal transcribed spacer (ITS) region is the...

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Bibliographic Details

Published in:	BMC Genomics
Main Authors:	Raf Winand, Elizabet D’hooge, Alexander Van Uffelen, Bert Bogaerts, Julien Van Braekel, Stefan Hoffman, Nancy H. C. J. Roosens, Pierre Becker, Sigrid C. J. De Keersmaecker, Kevin Vanneste
Format:	Article
Language:	English
Published:	BMC 2025-08-01
Subjects:	Fungal identification ITS Targeted metagenomics Illumina Metabarcoding Orientation
Online Access:	https://doi.org/10.1186/s12864-025-11917-y