Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655

Abstract Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E....

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Bibliographic Details

Published in:	AMB Express
Main Authors:	Xiaoliang He, Yuwen Ren, Wanli Meng, Xinran Yu, Xiaohui Zhou
Format:	Article
Language:	English
Published:	SpringerOpen 2020-09-01
Subjects:	Knocked out cpxP Crispr/Cas9 Homologous repair Escherichia coli
Online Access:	http://link.springer.com/article/10.1186/s13568-020-01099-z