Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens

The optimization of recombinant protein production in bacteria is an important stage of process development, especially for difficult-to-express proteins that are particularly sensitive or recalcitrant. The optimal expression level must be neither too low, which would limit yields, nor too high, whi...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Joel Eichmann, Markus Oberpaul, Tobias Weidner, Doreen Gerlach, Peter Czermak
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2019-10-01
Subjects:	Golden Gate MoClo protein secretion genetic engineering high-throughput screening antimicrobial peptide
Online Access:	https://www.frontiersin.org/article/10.3389/fbioe.2019.00254/full