Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.

Bibliographic Details
Published in:Nature Communications
Main Authors: Ioannis Mougiakos, Prarthana Mohanraju, Elleke F. Bosma, Valentijn Vrouwe, Max Finger Bou, Mihris I. S. Naduthodi, Alex Gussak, Rudolf B. L. Brinkman, Richard van Kranenburg, John van der Oost
Format: Article
Language:English
Published: Nature Portfolio 2017-11-01
Online Access:https://doi.org/10.1038/s41467-017-01591-4

Internet

https://doi.org/10.1038/s41467-017-01591-4

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