Development of a nanobody-based competitive enzyme-linked immunosorbent assay for the sensitive detection of antibodies against porcine deltacoronavirus

ABSTRACT Porcine deltacoronavirus (PDCoV) is an emerging porcine enteric coronavirus causing significant economic losses to the pig farming industry globally. In this study, we expressed the S protein of a highly virulent PDCoV strain in the CHO eukaryotic expression system. After immunizing alpaca...

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Bibliographic Details

Published in:	Journal of Clinical Microbiology
Main Authors:	Ruiming Yu, Liping Zhang, Yingjie Bai, Peng Zhou, Jun Yang, Dongsheng Wang, Liyang Wei, Zhongwang Zhang, Chenghua Yan, Yonglu Wang, Huichen Guo, Li Pan, Ligang Yuan, Xinsheng Liu
Format:	Article
Language:	English
Published:	American Society for Microbiology 2025-03-01
Subjects:	porcine deltacoronavirus nanobody cELISA
Online Access:	https://journals.asm.org/doi/10.1128/jcm.01615-24