Topoisomerase Inhibitors and PIM1 Kinase Inhibitors Improve Gene Editing Efficiency Mediated by CRISPR-Cas9 and Homology-Directed Repair

The CRISPR-Cas9 system has emerged as the most prevalent gene editing technology due to its simplicity, high efficiency, and low cost. However, the homology-directed repair (HDR)-mediated gene knock-in in this system suffers from low efficiency, which limits its application in animal model preparati...

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Bibliographic Details

Published in:	Molecules
Main Authors:	Ying Yun, Min Wang, Shimeng Guo, Xin Xie
Format:	Article
Language:	English
Published:	MDPI AG 2024-06-01
Subjects:	CRISPR-CAS9 gene editing homology-directed repair knock-in
Online Access:	https://www.mdpi.com/1420-3049/29/12/2890